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fgfr3 gene silencing  (TargetMol)


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    TargetMol fgfr3 gene silencing
    Figure 1. Up-regulation of <t>FGFR3</t> in renal pelvis tissues from Caucasian patients with urothelial carcinoma. Tissue microarray slides containing sections from Caucasian patients with renal pelvis urothelial carcinoma at stages T1–2 (n = 62), T3 (n = 17), and T4 (n = 5) were subjected to immuno- histochemistry (IHC) staining with FGFR3. (A) Representative FGFR3-positive stained images from stages T1–2, T3, and T4. (B) Quantification of FGFR3 expression levels from IHC staining results (scores were from 1 to 4). Data are shown in box plots displaying 25% to 75% percentile. * indicates significance between the indicated groups. (C) Heterogeneous distribution of FGFR3 in normal renal tubular epithelium. Note that proximal renal tubules (black arrows) usually show weak FGFR3 expression (score 1+), while distal tubules (white arrowheads) in the same specimen likely display stronger FGFR3 signals (score 2+~3+).
    Fgfr3 Gene Silencing, supplied by TargetMol, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgfr3+gene+silencing/pm36675289-275-5-23?v=TargetMol
    Average 91 stars, based on 1 article reviews
    fgfr3 gene silencing - by Bioz Stars, 2026-07
    91/100 stars

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    1) Product Images from "Genetic Interference of FGFR3 Impedes Invasion of Upper Tract Urothelial Carcinoma Cells by Alleviating RAS/MAPK Signal Activity."

    Article Title: Genetic Interference of FGFR3 Impedes Invasion of Upper Tract Urothelial Carcinoma Cells by Alleviating RAS/MAPK Signal Activity.

    Journal: International journal of molecular sciences

    doi: 10.3390/ijms24021776

    Figure 1. Up-regulation of FGFR3 in renal pelvis tissues from Caucasian patients with urothelial carcinoma. Tissue microarray slides containing sections from Caucasian patients with renal pelvis urothelial carcinoma at stages T1–2 (n = 62), T3 (n = 17), and T4 (n = 5) were subjected to immuno- histochemistry (IHC) staining with FGFR3. (A) Representative FGFR3-positive stained images from stages T1–2, T3, and T4. (B) Quantification of FGFR3 expression levels from IHC staining results (scores were from 1 to 4). Data are shown in box plots displaying 25% to 75% percentile. * indicates significance between the indicated groups. (C) Heterogeneous distribution of FGFR3 in normal renal tubular epithelium. Note that proximal renal tubules (black arrows) usually show weak FGFR3 expression (score 1+), while distal tubules (white arrowheads) in the same specimen likely display stronger FGFR3 signals (score 2+~3+).
    Figure Legend Snippet: Figure 1. Up-regulation of FGFR3 in renal pelvis tissues from Caucasian patients with urothelial carcinoma. Tissue microarray slides containing sections from Caucasian patients with renal pelvis urothelial carcinoma at stages T1–2 (n = 62), T3 (n = 17), and T4 (n = 5) were subjected to immuno- histochemistry (IHC) staining with FGFR3. (A) Representative FGFR3-positive stained images from stages T1–2, T3, and T4. (B) Quantification of FGFR3 expression levels from IHC staining results (scores were from 1 to 4). Data are shown in box plots displaying 25% to 75% percentile. * indicates significance between the indicated groups. (C) Heterogeneous distribution of FGFR3 in normal renal tubular epithelium. Note that proximal renal tubules (black arrows) usually show weak FGFR3 expression (score 1+), while distal tubules (white arrowheads) in the same specimen likely display stronger FGFR3 signals (score 2+~3+).

    Techniques Used: Microarray, Immunohistochemistry, Staining, Expressing

    Figure 2. Up-regulation of FGFR3 in renal pelvis tissues from Asian patients with upper tract urothelial carcinoma (UTUC). Renal pelvis tissues were collected from Asian patients diagnosed with UTUC at different TNM stages, including T1–2 (n = 44), T3 (n = 38), and T4 (n = 30). The formalin- fixed paraffin-embedded sections were subjected to immunohistochemistry (IHC) analysis of FGFR3 expression. (A) Representative FGFR3-positive stained images from stages T1–2, T3, and T4 and that of archived normal kidney pelvis tissues. (B) Analysis of FGFR3 IHC scoring results. (C) Statistical analysis of ethnic difference in FGFR3 expression at different UTUC stages. Data are shown in box plots displaying 25% to 75% percentiles. * indicates significance between the indicated groups.
    Figure Legend Snippet: Figure 2. Up-regulation of FGFR3 in renal pelvis tissues from Asian patients with upper tract urothelial carcinoma (UTUC). Renal pelvis tissues were collected from Asian patients diagnosed with UTUC at different TNM stages, including T1–2 (n = 44), T3 (n = 38), and T4 (n = 30). The formalin- fixed paraffin-embedded sections were subjected to immunohistochemistry (IHC) analysis of FGFR3 expression. (A) Representative FGFR3-positive stained images from stages T1–2, T3, and T4 and that of archived normal kidney pelvis tissues. (B) Analysis of FGFR3 IHC scoring results. (C) Statistical analysis of ethnic difference in FGFR3 expression at different UTUC stages. Data are shown in box plots displaying 25% to 75% percentiles. * indicates significance between the indicated groups.

    Techniques Used: Immunohistochemistry, Expressing, Staining

    Figure 3. Suppressive effect of FGFR3 gene silencing on proliferation of cultured UTUC cells. Two UTUC cell lines, BFTC-909 (A) and UM-UC-14 (B), were treated with FGFR3-targeted siRNA or scramble non-target control (NTC), followed by a WST-1-based proliferation assay. Optical den- sity (OD) measured is expressed as mean ± SEM (n = 3). *** p < 0.001 compared with respective NTC groups.
    Figure Legend Snippet: Figure 3. Suppressive effect of FGFR3 gene silencing on proliferation of cultured UTUC cells. Two UTUC cell lines, BFTC-909 (A) and UM-UC-14 (B), were treated with FGFR3-targeted siRNA or scramble non-target control (NTC), followed by a WST-1-based proliferation assay. Optical den- sity (OD) measured is expressed as mean ± SEM (n = 3). *** p < 0.001 compared with respective NTC groups.

    Techniques Used: Cell Culture, Control, Proliferation Assay

    Figure 4. Suppressive effect of FGFR3 gene silencing on migration of cultured UTUC cells. Two UTUC cell lines, BFTC-909 (A) and UM-UC-14 (B), were treated with FGFR3-targeted siRNA or scramble non-target control (NTC), followed by consecutive microscopic documentation for the post-wounding closure at the indicated time points.
    Figure Legend Snippet: Figure 4. Suppressive effect of FGFR3 gene silencing on migration of cultured UTUC cells. Two UTUC cell lines, BFTC-909 (A) and UM-UC-14 (B), were treated with FGFR3-targeted siRNA or scramble non-target control (NTC), followed by consecutive microscopic documentation for the post-wounding closure at the indicated time points.

    Techniques Used: Migration, Cell Culture, Control

    Figure 7. Effects of FGFR3 gene silencing and FGFR3 kinase inhibition on apoptosis of cultured UTUC cells. (A) BFTC-909 cells were treated with either FGFR3-targeted siRNA, scramble non-target control (NTC), or PD173074 at 10 nM for 48 h and subjected to TUNEL fluorescent staining. The TUNEL-positive apoptotic cells show green fluorescence, while DAPI blue fluorescence indicates nuclei of all cells. Original magnification: 100×. (B) Morphometry analysis on TUNEL-positive rates in the treated cells. Density data are expressed as mean ± SEM (n = 8). *** indicates p < 0.001 vs. control group. ND, not detectable.
    Figure Legend Snippet: Figure 7. Effects of FGFR3 gene silencing and FGFR3 kinase inhibition on apoptosis of cultured UTUC cells. (A) BFTC-909 cells were treated with either FGFR3-targeted siRNA, scramble non-target control (NTC), or PD173074 at 10 nM for 48 h and subjected to TUNEL fluorescent staining. The TUNEL-positive apoptotic cells show green fluorescence, while DAPI blue fluorescence indicates nuclei of all cells. Original magnification: 100×. (B) Morphometry analysis on TUNEL-positive rates in the treated cells. Density data are expressed as mean ± SEM (n = 8). *** indicates p < 0.001 vs. control group. ND, not detectable.

    Techniques Used: Inhibition, Cell Culture, Control, TUNEL Assay, Staining

    Figure 8. A mechanistic scheme depicting the effects of FGFR3 gene silencing on RAS/mitogen- activated protein kinase (MAPK) signaling and renal pelvis tumorigenesis. Upon stimulation by fibroblast growth factor (FGF), the intracellular tyrosine kinase domain of FGF receptor 3 (FGFR3) may deliver the mitogenic signal through RAS/MAPK cascade and downstream mediator activation, including extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). Hence, FGFR3 overexpression and activation may enhance expression of epithelial–mesenchymal transition (EMT) transcription factors and mediators, which eventually contribute to tumor progression. Our findings suggest that FGFR3 siRNA delivery is a useful strategy for alleviating the RAS/MAPK signaling axis and EMT marker expression in renal pelvis urothelial cells, thereby suppressing their metastatic activity and tumor progression.
    Figure Legend Snippet: Figure 8. A mechanistic scheme depicting the effects of FGFR3 gene silencing on RAS/mitogen- activated protein kinase (MAPK) signaling and renal pelvis tumorigenesis. Upon stimulation by fibroblast growth factor (FGF), the intracellular tyrosine kinase domain of FGF receptor 3 (FGFR3) may deliver the mitogenic signal through RAS/MAPK cascade and downstream mediator activation, including extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). Hence, FGFR3 overexpression and activation may enhance expression of epithelial–mesenchymal transition (EMT) transcription factors and mediators, which eventually contribute to tumor progression. Our findings suggest that FGFR3 siRNA delivery is a useful strategy for alleviating the RAS/MAPK signaling axis and EMT marker expression in renal pelvis urothelial cells, thereby suppressing their metastatic activity and tumor progression.

    Techniques Used: Activation Assay, Over Expression, Expressing, Marker, Activity Assay



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    fgfr3 gene silencing  (TargetMol)
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    TargetMol fgfr3 gene silencing
    Figure 1. Up-regulation of <t>FGFR3</t> in renal pelvis tissues from Caucasian patients with urothelial carcinoma. Tissue microarray slides containing sections from Caucasian patients with renal pelvis urothelial carcinoma at stages T1–2 (n = 62), T3 (n = 17), and T4 (n = 5) were subjected to immuno- histochemistry (IHC) staining with FGFR3. (A) Representative FGFR3-positive stained images from stages T1–2, T3, and T4. (B) Quantification of FGFR3 expression levels from IHC staining results (scores were from 1 to 4). Data are shown in box plots displaying 25% to 75% percentile. * indicates significance between the indicated groups. (C) Heterogeneous distribution of FGFR3 in normal renal tubular epithelium. Note that proximal renal tubules (black arrows) usually show weak FGFR3 expression (score 1+), while distal tubules (white arrowheads) in the same specimen likely display stronger FGFR3 signals (score 2+~3+).
    Fgfr3 Gene Silencing, supplied by TargetMol, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fgfr3+gene+silencing/pm36675289-275-5-23?v=TargetMol
    Average 91 stars, based on 1 article reviews
    fgfr3 gene silencing - by Bioz Stars, 2026-07
    91/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 1. Up-regulation of FGFR3 in renal pelvis tissues from Caucasian patients with urothelial carcinoma. Tissue microarray slides containing sections from Caucasian patients with renal pelvis urothelial carcinoma at stages T1–2 (n = 62), T3 (n = 17), and T4 (n = 5) were subjected to immuno- histochemistry (IHC) staining with FGFR3. (A) Representative FGFR3-positive stained images from stages T1–2, T3, and T4. (B) Quantification of FGFR3 expression levels from IHC staining results (scores were from 1 to 4). Data are shown in box plots displaying 25% to 75% percentile. * indicates significance between the indicated groups. (C) Heterogeneous distribution of FGFR3 in normal renal tubular epithelium. Note that proximal renal tubules (black arrows) usually show weak FGFR3 expression (score 1+), while distal tubules (white arrowheads) in the same specimen likely display stronger FGFR3 signals (score 2+~3+).

    Journal: International journal of molecular sciences

    Article Title: Genetic Interference of FGFR3 Impedes Invasion of Upper Tract Urothelial Carcinoma Cells by Alleviating RAS/MAPK Signal Activity.

    doi: 10.3390/ijms24021776

    Figure Lengend Snippet: Figure 1. Up-regulation of FGFR3 in renal pelvis tissues from Caucasian patients with urothelial carcinoma. Tissue microarray slides containing sections from Caucasian patients with renal pelvis urothelial carcinoma at stages T1–2 (n = 62), T3 (n = 17), and T4 (n = 5) were subjected to immuno- histochemistry (IHC) staining with FGFR3. (A) Representative FGFR3-positive stained images from stages T1–2, T3, and T4. (B) Quantification of FGFR3 expression levels from IHC staining results (scores were from 1 to 4). Data are shown in box plots displaying 25% to 75% percentile. * indicates significance between the indicated groups. (C) Heterogeneous distribution of FGFR3 in normal renal tubular epithelium. Note that proximal renal tubules (black arrows) usually show weak FGFR3 expression (score 1+), while distal tubules (white arrowheads) in the same specimen likely display stronger FGFR3 signals (score 2+~3+).

    Article Snippet: To compare the effect of FGFR3 gene silencing with that of receptor kinase inhibition, the cells were treated with PD173074 (Cat. No. T2642, Targetmol, Wellesley Hills, MA, USA) at 10 nM.

    Techniques: Microarray, Immunohistochemistry, Staining, Expressing

    Figure 2. Up-regulation of FGFR3 in renal pelvis tissues from Asian patients with upper tract urothelial carcinoma (UTUC). Renal pelvis tissues were collected from Asian patients diagnosed with UTUC at different TNM stages, including T1–2 (n = 44), T3 (n = 38), and T4 (n = 30). The formalin- fixed paraffin-embedded sections were subjected to immunohistochemistry (IHC) analysis of FGFR3 expression. (A) Representative FGFR3-positive stained images from stages T1–2, T3, and T4 and that of archived normal kidney pelvis tissues. (B) Analysis of FGFR3 IHC scoring results. (C) Statistical analysis of ethnic difference in FGFR3 expression at different UTUC stages. Data are shown in box plots displaying 25% to 75% percentiles. * indicates significance between the indicated groups.

    Journal: International journal of molecular sciences

    Article Title: Genetic Interference of FGFR3 Impedes Invasion of Upper Tract Urothelial Carcinoma Cells by Alleviating RAS/MAPK Signal Activity.

    doi: 10.3390/ijms24021776

    Figure Lengend Snippet: Figure 2. Up-regulation of FGFR3 in renal pelvis tissues from Asian patients with upper tract urothelial carcinoma (UTUC). Renal pelvis tissues were collected from Asian patients diagnosed with UTUC at different TNM stages, including T1–2 (n = 44), T3 (n = 38), and T4 (n = 30). The formalin- fixed paraffin-embedded sections were subjected to immunohistochemistry (IHC) analysis of FGFR3 expression. (A) Representative FGFR3-positive stained images from stages T1–2, T3, and T4 and that of archived normal kidney pelvis tissues. (B) Analysis of FGFR3 IHC scoring results. (C) Statistical analysis of ethnic difference in FGFR3 expression at different UTUC stages. Data are shown in box plots displaying 25% to 75% percentiles. * indicates significance between the indicated groups.

    Article Snippet: To compare the effect of FGFR3 gene silencing with that of receptor kinase inhibition, the cells were treated with PD173074 (Cat. No. T2642, Targetmol, Wellesley Hills, MA, USA) at 10 nM.

    Techniques: Immunohistochemistry, Expressing, Staining

    Figure 3. Suppressive effect of FGFR3 gene silencing on proliferation of cultured UTUC cells. Two UTUC cell lines, BFTC-909 (A) and UM-UC-14 (B), were treated with FGFR3-targeted siRNA or scramble non-target control (NTC), followed by a WST-1-based proliferation assay. Optical den- sity (OD) measured is expressed as mean ± SEM (n = 3). *** p < 0.001 compared with respective NTC groups.

    Journal: International journal of molecular sciences

    Article Title: Genetic Interference of FGFR3 Impedes Invasion of Upper Tract Urothelial Carcinoma Cells by Alleviating RAS/MAPK Signal Activity.

    doi: 10.3390/ijms24021776

    Figure Lengend Snippet: Figure 3. Suppressive effect of FGFR3 gene silencing on proliferation of cultured UTUC cells. Two UTUC cell lines, BFTC-909 (A) and UM-UC-14 (B), were treated with FGFR3-targeted siRNA or scramble non-target control (NTC), followed by a WST-1-based proliferation assay. Optical den- sity (OD) measured is expressed as mean ± SEM (n = 3). *** p < 0.001 compared with respective NTC groups.

    Article Snippet: To compare the effect of FGFR3 gene silencing with that of receptor kinase inhibition, the cells were treated with PD173074 (Cat. No. T2642, Targetmol, Wellesley Hills, MA, USA) at 10 nM.

    Techniques: Cell Culture, Control, Proliferation Assay

    Figure 4. Suppressive effect of FGFR3 gene silencing on migration of cultured UTUC cells. Two UTUC cell lines, BFTC-909 (A) and UM-UC-14 (B), were treated with FGFR3-targeted siRNA or scramble non-target control (NTC), followed by consecutive microscopic documentation for the post-wounding closure at the indicated time points.

    Journal: International journal of molecular sciences

    Article Title: Genetic Interference of FGFR3 Impedes Invasion of Upper Tract Urothelial Carcinoma Cells by Alleviating RAS/MAPK Signal Activity.

    doi: 10.3390/ijms24021776

    Figure Lengend Snippet: Figure 4. Suppressive effect of FGFR3 gene silencing on migration of cultured UTUC cells. Two UTUC cell lines, BFTC-909 (A) and UM-UC-14 (B), were treated with FGFR3-targeted siRNA or scramble non-target control (NTC), followed by consecutive microscopic documentation for the post-wounding closure at the indicated time points.

    Article Snippet: To compare the effect of FGFR3 gene silencing with that of receptor kinase inhibition, the cells were treated with PD173074 (Cat. No. T2642, Targetmol, Wellesley Hills, MA, USA) at 10 nM.

    Techniques: Migration, Cell Culture, Control

    Figure 7. Effects of FGFR3 gene silencing and FGFR3 kinase inhibition on apoptosis of cultured UTUC cells. (A) BFTC-909 cells were treated with either FGFR3-targeted siRNA, scramble non-target control (NTC), or PD173074 at 10 nM for 48 h and subjected to TUNEL fluorescent staining. The TUNEL-positive apoptotic cells show green fluorescence, while DAPI blue fluorescence indicates nuclei of all cells. Original magnification: 100×. (B) Morphometry analysis on TUNEL-positive rates in the treated cells. Density data are expressed as mean ± SEM (n = 8). *** indicates p < 0.001 vs. control group. ND, not detectable.

    Journal: International journal of molecular sciences

    Article Title: Genetic Interference of FGFR3 Impedes Invasion of Upper Tract Urothelial Carcinoma Cells by Alleviating RAS/MAPK Signal Activity.

    doi: 10.3390/ijms24021776

    Figure Lengend Snippet: Figure 7. Effects of FGFR3 gene silencing and FGFR3 kinase inhibition on apoptosis of cultured UTUC cells. (A) BFTC-909 cells were treated with either FGFR3-targeted siRNA, scramble non-target control (NTC), or PD173074 at 10 nM for 48 h and subjected to TUNEL fluorescent staining. The TUNEL-positive apoptotic cells show green fluorescence, while DAPI blue fluorescence indicates nuclei of all cells. Original magnification: 100×. (B) Morphometry analysis on TUNEL-positive rates in the treated cells. Density data are expressed as mean ± SEM (n = 8). *** indicates p < 0.001 vs. control group. ND, not detectable.

    Article Snippet: To compare the effect of FGFR3 gene silencing with that of receptor kinase inhibition, the cells were treated with PD173074 (Cat. No. T2642, Targetmol, Wellesley Hills, MA, USA) at 10 nM.

    Techniques: Inhibition, Cell Culture, Control, TUNEL Assay, Staining

    Figure 8. A mechanistic scheme depicting the effects of FGFR3 gene silencing on RAS/mitogen- activated protein kinase (MAPK) signaling and renal pelvis tumorigenesis. Upon stimulation by fibroblast growth factor (FGF), the intracellular tyrosine kinase domain of FGF receptor 3 (FGFR3) may deliver the mitogenic signal through RAS/MAPK cascade and downstream mediator activation, including extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). Hence, FGFR3 overexpression and activation may enhance expression of epithelial–mesenchymal transition (EMT) transcription factors and mediators, which eventually contribute to tumor progression. Our findings suggest that FGFR3 siRNA delivery is a useful strategy for alleviating the RAS/MAPK signaling axis and EMT marker expression in renal pelvis urothelial cells, thereby suppressing their metastatic activity and tumor progression.

    Journal: International journal of molecular sciences

    Article Title: Genetic Interference of FGFR3 Impedes Invasion of Upper Tract Urothelial Carcinoma Cells by Alleviating RAS/MAPK Signal Activity.

    doi: 10.3390/ijms24021776

    Figure Lengend Snippet: Figure 8. A mechanistic scheme depicting the effects of FGFR3 gene silencing on RAS/mitogen- activated protein kinase (MAPK) signaling and renal pelvis tumorigenesis. Upon stimulation by fibroblast growth factor (FGF), the intracellular tyrosine kinase domain of FGF receptor 3 (FGFR3) may deliver the mitogenic signal through RAS/MAPK cascade and downstream mediator activation, including extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). Hence, FGFR3 overexpression and activation may enhance expression of epithelial–mesenchymal transition (EMT) transcription factors and mediators, which eventually contribute to tumor progression. Our findings suggest that FGFR3 siRNA delivery is a useful strategy for alleviating the RAS/MAPK signaling axis and EMT marker expression in renal pelvis urothelial cells, thereby suppressing their metastatic activity and tumor progression.

    Article Snippet: To compare the effect of FGFR3 gene silencing with that of receptor kinase inhibition, the cells were treated with PD173074 (Cat. No. T2642, Targetmol, Wellesley Hills, MA, USA) at 10 nM.

    Techniques: Activation Assay, Over Expression, Expressing, Marker, Activity Assay